1. Total nitrogen SSC - 26.3, 38.2.2. - H2SO4 - mixing accelerator - distillation. Parallel determination of < 0.005%. Organic nitrogen compounds in plant samples by - H2SO4 - mixing accelerator boiled decomposition, elimination of nitrogen into ammonium salt, can through the basification, distillation, standard acid titration nitrogen.
　　2. Total phosphorus SSC - 26.4. Vanadium molybdate yellow colorimetric method. Parallel determination of < 0.005%. Use - H2SO4 - H2O2 oxidizing boiled plant samples, the dehydration carbonized, oxidative decomposition of organic matter, organic nitrogen and phosphorus into the ammonium salt and phosphate, the fluid of phosphorus under test and the partial vanadate molybdate and under acid condition can generate yellow ternary molybdenum heteropoly acid vanadium phosphate, available colorimetric method.
　　3. Total potassium SSC - 26.5. The flame photometer method. Potassium in plants with ion mode, sample processing is relatively simple, commonly used in the actual work - H2SO4 - H2O2 boil after the plant samples, at the same time for the determination of nitrogen, phosphorus and potassium, potassium of flame photometer method
　　4. All the calcium and magnesium in SSC - 27.3 for all after pretreatment, using plasma atomic emission spectroscopy （icp-aes） method determination. With HNO3 and HClO4 boiled plant samples, the oxidation of organic matter and calcium and magnesium ions into state, and then use the ICP method of determination of calcium and magnesium content in boiling fluid elimination.
　　5. Copper, iron, manganese and zinc SSC - 28.5.3. Atomic absorption spectrophotometry. With HNO3 and HClO4 boiled plant samples, then boil with atomic absorption spectrophotometry and copper, iron, manganese and zinc content in the liquid.
　　6. Total sulfur SSC - 27.4.2.2. Plasma emission spectrometry （ICP）。 With HNO3 and HClO4 boiled plant samples, and then use the ICP method of determination of sulfur content in boiling fluid elimination.
　　7. The SSC - 38.3.2 amino acids. Amino acid analyzer. When mobile phase （buffer solution） to promote amino acids through the chromatographic column with cation exchange resin, amino acid and the exchange of groups for ion exchange resin, when using different pH buffer solution, because of the different exchange capacity and separate amino acid mixture, most of the outflow of chromatographic column amino acid reacts with indene three ketones under 100 ℃ generated purple material, the material in the 570 - nm wavelength absorption peaks of the largest, but by the spectrophotometer detection; But the proline and hydroxyproline and indene three ketone generate yellow substance, one absorption peak at 440 nm wavelength, can also be used by the spectrophotometer detection, after the recorder and data processor to get all kinds of amino acid content.